Myc western blot
WebMyc-p65-V5 (Lane 3-6), using Anti-Myc Tag Polyclonal Antibody (Product # MA1-980-1MG) in Western Blot. This product has been shown to detect Myc Tag at both N- and C- … Web18 jul. 2024 · So it is convenient to detect and identify FLAG fusion proteins by Western Blot, ELISA and other methods. His6-tag. His6-tag, 6 histidines bound by a nickel or cobalt chelate (HHHHHH), which can be inserted at the C-terminus or N-terminus of the target protein. They have two uses as tags, one is to form an epitope to facilitate purification …
Myc western blot
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Web6 mei 2016 · Western Blot Antibodies Most recent answer 5th May, 2024 Debanjan Bhattacharya University of Cincinnati I have had similar experience with western blot … WebC-Myc 标签蛋白，是一个含11个氨基酸的小标签，这11个氨基酸作为抗原表位表达在不同的蛋白质框架中仍可识别其相应抗体。 C-Myc tag已成功应用在 Western-blot杂交技术、免疫沉淀和流式细胞计量术中, 可用于检测 …
Web9 dec. 2024 · (E) Western blot analysis of p38 MAPK phosphorylation levels in PyVT tumor cells and MCF-7 cells deficient of α5(IV) or DDR1. (F) Western blot analysis of c-Myc, glucose transporter, and glycolytic enzyme levels in PyVT tumor cells subjected to p38 MAPK inhibitor SB203580 treatment. WebThe c-Myc protein is a 62 kDa transcription factor that is encoded by the c-Myc gene on human chromosome 8q24. c-Myc is commonly activated in a variety of tumor cells and …
WebWestern blot analysis of extracts from HeLa, BaF3 and NBT-11 cells, using c-Myc Antibody. Simple Western™ analysis of lysates (1 mg/mL) from HeLa cells using c-Myc … The scientists at CST are excited to work with you to tailor products to meet the … *Terms and Conditions. Guarantee applies to catalog or off the shelf orders of stand … In this digital library, you will find resources to help guide you through your … This page will let you add multiple products by manual entry. NOTE: Many of our … Quickly identify your next research target using the interactive pathways, … KinomeView ® Service - Western blot pre-screen to identify ideal enrichment … Doing good science is important. So is just doing good. Cell Signaling Technology … Cell Signaling Technology antibodies are validated in-house for Western Blot, … WebDownload scientific diagram Western blot analysis of the N-myc protein in medulloblastoma tissue using an anti-N-myc antiserum (OA:11:803). Lane A, illustration of medulloblastoma extract ...
WebOverexpression of c-Myc Can Rescue the Decreased Proliferation of U87 Cells Induced by PCGF1 Knock-Down. To verify the inhibitory effect on c-Myc and proliferation, caused by PCGF1 knockdown, we transfected the c-Myc plasmid into KD cells. The transfection efficiency was confirmed by Western blot analysis .
WebThe expression of c-MYC was measured using RT-qPCR and western blot assays. In addition, luciferase activity assays were performed for the c-MYC promoters P1 and P2 using recombinant... dino\u0027s gyros greek cafeWebWestern blot analysis of extracts from HeLa cells, mock transfected or transfected with SignalSilence ® c-Myc siRNA I #6341, using c-Myc (D84C12) Rabbit mAb. Show Less … dino\u0027s hanover parkWebThe expression levels of c-Myc and apoptosis-related proteins, including pro-PARP, cleaved-PARP, pro-Caspase 3, and cleaved-Caspase 3, were confirmed in the cells by Western blotting. Additionally, the expression level of β-actin, which is used as an internal control, was also checked to confirm whether the equivalent protein was loaded. dino\u0027s holyokeWebThe c-myc tag is derived from the c-terminal of the c-myc gene and it can be effectively recognized by antibodies. As such, it is a widely used tag for the detection of proteins in applications such as western blot, immunoprecipitation, and flow cytometry. Purified c-myc tagged proteins have been successfully crystallized 3. beauty salon sakuraWeb11 jan. 2005 · MYCN was detected as described in Western blot analysis. ChIP. ChIP cloning was performed with lysates of the JF MYCN -amplified neuroblastoma cell line. Using a modified version of published protocols ( 19, 20 ), we performed two rounds of immunoprecipitation with an anti-MYCN mAb. dino\u0027s gyros greek cafe and tavernaWebMyc transcription factors are considered as the main oncoproteins responsible for glutamine addiction of tumor cells [22]. c-Myc drives glutamine uptake and catabolism by activating the expression of genes involved in glutamine metabolism, including glutaminase, and … dino\u0027s holyoke maWeb18 uur geleden · A western blot is a laboratory method used to detect specific protein molecules from among a mixture of proteins. This mixture can include all of the proteins … beauty salon san bernardino