Flag chip antibody

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August undefined, 2024

WebLane 1 is 10% input, lane 2 is HA-Tag (C29F4) Rabbit mAb, and lane 3 is Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900. Western blot was performed using HA-Tag (C29F4) Rabbit mAb. A conformation specific secondary antibody was used to avoid reactivity with IgG. Immunohistochemical analysis of paraffin-embedded COS cells, untransfected (right ... WebThis antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. Storage Supplied in 10 mM sodium HEPES (pH 7.5), …

DYKDDDDK Tag (9A3) Mouse mAb (Binds to same epitope as Sigma

WebChIP Assay: Mus musculus: 150 μl: Sigma-Aldrich, cat. no. A2220: affinity gel: M2 wash buffer: Chromatin tandem affinity purification sequencing: Western Blot: Sigma-Aldrich, … WebAnti-HA tag antibody - ChIP Grade See all HA tag primary antibodies Description Rabbit polyclonal to HA tag - ChIP Grade Host species Rabbit Specificity ELISA: The anti HA diluted 1:70.000 gave an O.D.=1.0 in a 15 … portrait of a young flavian woman

Antibody for the detection of FLAG™ (200-301-383) Rockland

WebEpitope tags, such as c-myc and HA, are short sequences that are fused onto proteins and are frequently used in antibody-based assays. Epitope tags are generally shorter in length than affinity tags and are, therefore, less likely to affect protein function 1. Although they can be used for affinity purification, the columns are based upon ... WebA FLAG tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG tag to this protein … WebApr 8, 2024 · The lysates were subsequently incubated with IgG or ChIP-grade antibody against FOXA2 at 4 °C overnight and then incubated with Protein A + G Agarose/Salmon Sperm DNA at 4 °C for 3 h. After washing with low salt and high salt buffer, elution, and reverse cross-linking, the DNA was added with EDTA, Tris pH 6.5, and proteinase K at … optomacinemaxp2shortthrowprojector

HA-Tag (C29F4) Rabbit mAb Cell Signaling Technology

Genome-wide (ChIP-seq) identification of target genes regulated …

WebSpecificity / Sensitivity. GFP Antibody detects GFP, YFP, and CFP-tagged proteins exogenously expressed in cells. This antibody does not detect RFP-tagged proteins. Please note that the GFP, YFP, and CFP tags add approximately 27 kDa to the molecular weight of the fusion protein. optomany occWebHA Tag Mouse Monoclonal Antibody: ... Immunohistochemistry; ICC, Immunocytochemistry; FC, Flow Cytometry; ChIP, Chromatin Immunoprecipitation Assay;ChIP-seq, ChIP-sequencing. ... Flag Tag Mouse Monoclonal Antibody (HRP Conjugated) 50μl/200μl/1ml: AF2858: HA Tag Mouse Monoclonal Antibody: … portrait of a young venetian woman

"WebDYKDDDDK Epitope Tag Antibody - ChIP assays were performed using Plasmodium falciparium cells stably expressing DYKDDDDK-Ty1-H2A (blue bars) and wild type Plasmodium falciparium cells (yellow bars). Primer pairs were used for active genes, inactive genes, and variable genes. Sheared chromatin from 1x106 cells and 10 ug of antibody … " - Flag chip antibody

Flag chip antibody

VprBP/DCAF1 regulates p53 function and stability through site …

WebJan 11, 2013 · The sheared samples were diluted into 0.9 ml of ChIP dilution buffer and precleared with Protein G-agarose/salmon sperm DNA beads for 1 h. Subsequently, immunoprecipitation was conducted with anti-FLAG antibody (to precipitate XBP1s and XBP1u and their bound chromatin) or XBP1 antibody (Santa Cruz Biotechnology, Inc.) … http://www.cmctag.com/products/DYKDDDDK-FLAG-tag-rabbit-mab-Epitope-Tag-Antibody-ChIP-Grade-AT1748.html

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WebGeneral description. Anti-FLAG M2 Magnetic Beads are 4% agarose beads bound with the Anti-FLAG M2 (mouse monoclonal) antibody. The M2 antibody recognizes the FLAG sequence at the N-terminus, Met-N-terminus and C-terminus. This alows for detection and capture of fusion proteins containing a FLAG peptide sequence. WebChIP was performed using Histone H3K4me1 Recombinant Rabbit Polyclonal Antibody (Cat. No. 710795, 5 µg) on sheared chromatin from 2 million Jurkat cells. Normal Rabbit IgG was used as a negative IP …

WebA Flag®-tagged protein can be immunoprecipitated from a crude cellular extract using an antibody bound to the Flag®-tag, an anti-Flag® antibody. As Flag® is a registered trademark, anti-Flag® antibodies are often called DYKDDDDK antibodies. Isolation of a Flag®-tagged protein from a crude sample. WebEMT-activated secretory and endocytic vesicular trafficking programs underlie a vulnerability to PI4K2A antagonism in lung cancer. In The Journal of Clinical Investigation on 3 April 2024 by Tan, X., Xiao, G. Y., et al..

WebPierce Protein Methods. Immunoprecipitation (IP) is the small-scale affinity purification of antigens using a specific antibody that is immobilized to a solid support such as magnetic particles or agarose resin. Immunoprecipitation is one of the most widely used methods for isolation of proteins and other biomolecules from cell or tissue ... WebImmunoprecipitation ( IP) is the technique of precipitating a protein antigen out of solution using an antibody that specifically binds to that particular protein. This process can be used to isolate and concentrate a particular protein from a sample containing many thousands of different proteins. Immunoprecipitation requires that the antibody ...

WebGeneral description. The ANTI-FLAG M2 mouse, affinity purified monoclonal antibody binds to fusion proteins containing a FLAG peptide …

WebFull-text available. Jan 2003. Igor N Berezovsky. Alla Kirzhner. Valery M Kirzhner. Edward N. Trifonov. During the last 30 years of protein research, the main emphasis has been given to ... portrait of a woman used by godWebAnti-FLAG Flow Cytometry Antibody Products. Products (73) User Reviews (6) Company View. Product View. Your search returned 73 FLAG Flow Cytometry Antibodies across … optomany q25WebANTI-FLAG® High Sensitivity, M2 coated 96-well plates. Synonym (s): Monoclonal ANTI-FLAG® M2 antibody produced in mouse, Anti-ddddk, Anti-dykddddk. Compare. Product No. portrait of an achiever abraham posterWebActive Motif's Tag-ChIP-IT ® Kit offers a method to perform ChIP without the need for protein-specific antibodies. Simply create an expression construct containing the protein … optomany login occWebCaco-2-N-flag and shRNA-Hsp90β-Caco-2-N-flag cells were infected with SARS-CoV-2 GFP/ΔN virus at MOI = 1 for 8 h, and cells were lysed with lysis buffer and 300 μL of supernatant was incubated with 4 μg of monoclonal ANTI-FLAG® M2 antibody overnight at 4 °C, followed by incubation with 30 μL of pre-washed Dynabeads™ Protein G at 4 °C ... portrait of a young indianWebThis antibody is a rabbit polyclonal antibody raised against 3xFlag (3xDYKDDDDKT) sequence and recognizes the (1x) and … portrait of an unknown woman amazonWebFeb 2, 2024 · Myc-SMAD3 was co-expressed with the Flag-tagged AR fragments (N-terminal transactivation domain, N-TAD; DNA binding domain, DBD; Ligand-binding domain, LBD) in 293T cells, and analyzed as described in (B). (D) Heatmap showing two replicates of AR ChIP-seq peaks relative to IgG negative control antibody. (E) AR ChIP-seq portrait of a young joan didion