WebDec 16, 2024 · To verify loss of calcium transients in neurons and Müller glia following BAPTA-AM application, we include imaging results from PDGFRα;cyto-GCaMP6f flox mice (PDGFRα-Cre: strain 013148), which label Müller glia and a subset of neurons with cytosolic GCaMP6f, as well as results from Slc1a3-CreER;Lck-GCaMP6f flox retinas (Figure … WebLck-GCaMP6f is a detector of single neuronal action potentials with fast response kinetics. It has an Lck-tag to improve its ability to reveal calcium signals in near membrane regions and fine processes ( e.g. , astrocyte processes). After Cre exposure, bright GCaMP6f fluorescence is observed following calcium binding (such as neuronal ...
Protocol to downregulate GABAergic-astrocyte signaling via …
WebSep 1, 2024 · A craniotomy was made to expose the visual cortex, and AAV2/5-gfaABC1D-cyto-GCaMP6f was injected to transduce cortical astrocytes for visualization of astrocytic Ca 2+ signals. Two-photon imaging of basal astrocyte Ca 2+ activity was conducted through a cranial window, 3 to 4 weeks later. WebCyto-GCaMP6f expression when driven by Aldh1l1- Cre/ERT2 in Ai95 mice at ~P66 was dependent on the tamoxifen dose administered in vivo. A. Representative images showing cyto-GCaMP6f expression as a function of tamoxifen dose in hippocampal CA1, V1 visual cortex and striatal brain sections. B. simplify summation
New Transgenic Mouse Lines for Selectively Targeting
WebJul 11, 2024 · Virus & Injection Primary Virus pZac2.1 gfaABC1D-cyto-GCaMP6f (AAV5) Promoter GFAP Injected Titer 5.00E+12 GC/ml Injected Volume 1μl Injection Rate 0.07 μl/min WebJul 11, 2024 · pZac2.1 gfaABC1D-cyto-GCaMP6f in Mouse (AAV5) Addgene #52925-AAV5. Caption:Olympus Confocal microscopy, 488nm laser, XLPLN25XWMP2. Data … WebTwo 300nL injections (600nL total virus) of AAV5-GFaABC1D.cyto-GCaMP6f were made into visual cortex (0.5–1.0mm anterior and 1.75–2.5mm lateral of bregma) at a depth of 0.2–0.3mm and 0.5mm from the pial surface, respectively. Virus was injected at a rate of 2nL/s, with a 10min wait following each injection to allow for diffusion. raymour flanigan lighting