Chimeric repressor gene-silencing technology
WebJan 30, 2024 · Introduction. Silencing gene expression, protein-coding or non-coding DNA, is a very important tool to interrogate the function of DNA. It has been reported that, with the guidance of customized single guide RNA, the catalytically inactivated dead Cas9 (dCas9) could bind to targeted promoter region to disrupt the transcriptional initiation and … WebMasaru Ohme-Takagi. Chimeric repressor gene-silencing technology (CRES-T) is a powerful tool that has recently been developed for the functional analysis of plant transcription factors and for the ...
Chimeric repressor gene-silencing technology
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WebOct 21, 2016 · Chimeric repressor gene silencing technology (CRES‐T) is a novel gene silencing system in which a dominant negative repressor of a TF suppresses the target genes of endogenous TF and its functionally redundant TFs (Hiratsu et al., 2003). WebSep 21, 2016 · In CRES-T, overexpression of a chimeric repressor, composed of the coding sequence of a transcription factor (TF) and short peptide designated as the repression domain, could interfere with the action of endogenous TF in plants.
WebGuided by chimeric sgRNA, engineered Cas12b facilitates robust genome editing in human cells and mice ... (NMD), thus silencing gene expression (130, 131). Gene expression can also be shut down by ABE-mediated destruction ... Advances in CRISPR/Cas gene-editing technology have led to new therapeutic options for a wide range of genetic and ... WebChimeric REpressor gene-Silencing Technology (CRES-T) was developed as a specific technology for gene silencing, mainly used for analyzing the function of plant …
WebDec 5, 2013 · We produced transgenic Arabidopsis plants that express chimeric genes for transcription factors converted to dominant repressors, using Chimeric REpressor gene … WebDedicated and highly accomplished Scientist & Inventor offering 30+ years of experience at state-of-the-art research facilities conducting sophisticated research in the development of new ...
WebApr 11, 2024 · Yeasts have been a part of human life since ancient times in the fermentation of many natural products used for food. In addition, in the 20th century, they became powerful tools to elucidate the functions of eukaryotic cells as soon as the techniques of molecular biology developed. Our molecular understandings of metabolism, cellular …
WebJan 25, 2006 · We have recently developed a novel gene silencing technology, called Chimeric REpressor Gene-Silencing Technology (CRES-T), in which a chimeric repressor is produced by fusion of a transcription factor to the plant-specific ERF associated Amphiphilic Repression (EAR)-like motif repression domain (Hiratsu et al., … china belt over roller conveyorWebMay 29, 2024 · Repressor element 1 silencing transcription factor (REST) is a neuronal repressor gene that regulates neuronal stem cell differentiation [11,12]. REST also plays a multifunctional role in the regulation of non-neurogenic cells [ 13 , 14 ]. china bend folding machine manufacturerWebFeb 22, 2014 · In the Chimeric repressor gene silencing technology (CRES-T) system (Hiratsu et al. 2003; Mitsuda et al. 2011 ), fusion of a transcription factor to the plant … china benchtop drying ovenWebDec 19, 2024 · Silencing of the JA synthetic gene SlAOC results in early flower abortion, ... In addition, overexpression of AtMYB24-SRDX (a chimeric repressor of SlMYB21) in tomato delays flower opening, ... and the Beijing Natural Science Foundation project-Key Project of Science and Technology Plan of Beijing Education Commission … china bend folding machine suppliersWebJan 1, 2011 · Chimeric REpressor gene Silencing Technology (CRES-T) is a useful tool for functional analysis of plant transcription factors. In this system, a chimeric repressor … china belt tracking rollerWebJan 1, 2016 · A modified version of the EAR domain, called SRDX (LDL DLE LRL GFA), has been used to create chimeric repressor gene silencing technology (CRES-T; Mitsuda et al., 2011). SRDX has the advantage that it is a short amino acid sequence that can be fused to many different transcription factors to obtain a strong repressive form. china belt dryer machine supplierWebAug 3, 2024 · For explaining the molecular mechanism of two-layered petaloid tepal development, the modified ABC model has been proposed, in which B class genes … china bendable stainless steel